Facilities & equipment
Principles of the 3R’s
We follow the principle of the 3Rs in our studies: replacement, reduction and refinement. Carefully drafted study designs, statistics and pilot studies to make sure the proper care and use of animals. Continuous monitoring and assessment are done in each study to ensure the wellbeing of animals at all times (in accordance with FELASA requirements.)
Our facilities include two state-of-the-art cell culture laboratories equipped with multiple incubators and laminar hoods for sterile work. One laboratory is dedicated to primary cell culture and another laboratory is for assays conducted with cell lines. The staff is trained for sterile cell culture work and only the trained personnel has access to the culture spaces.
Our in-house cell line portfolio includes several authenticated cell lines, but we are also cultivating a large variety of cells from our customers. Therefore, we have a very vast experience with different cell types and culture methods. We culture cells also for our in-house in vivo animal experiments and prepare the cells ready to be introduced to the animals. All the cells for animal experiments are tested for pathogens prior to start of the in-life phase.
Our cell culture work includes versatile cancer and bone cell assays, from cancer cell migration and viability assays to osteoblast and osteoclast experiments. We also perform co-cultures, for example combining immune cells with cancer cells. While in culture, labelled cells can be monitored with EVOS cell imaging system, and the growth of cells can be observed in real-time with a live cell imager Incucyte. Cells can also be subjected to different stainings after fixation followed by microscopic analysis. Also, expression studies can be performed after termination of cultures.
In vitro laboratories
Our in vitro laboratories host several histological and molecular biology activities. We perform immunohistochemistry staining followed by analysis. The analysed tissues come from our in vivo studies or directly from our customers. We have special expertise in bone analysis.
We also perform ELISA analysis of serum, plasma and cell culture samples. Multiplate readers allow measuring several samples in parallel. For example, bone turnover markers can be measured. In addition to samples obtained from animals and cells, we measure markers of clinical blood samples with IDS iSYS Multi-Discipline Automated System that allows automatic record of each sample and thus helps to accurately track even big number of samples.
Samples for different expression analysis are also prepared in-house. We can stain cell or tissue samples for flow cytometric analysis and extract RNA for further expression studies.