Pharmatest
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Breast cancer in vivo models
Pharmatest’s breast cancer models are fast and highly predictive models for translational cancer research. We offer three types of breast cancer models. These include intracardiac, intratibial, and orthotopic models, designed to study various aspects of cancer morphology such as primary tumor growth, invasion, and metastasis.
These models serve as essential tools for investigating the intricacies of tumor biology, drug responses, and metastatic behavior.
Importance of tumor microenvironment
Orthotopic breast cancer models provide a suitable platform for assessing the efficacy of drug compounds against primary tumor growth within the proper tissue microenvironment. They involve inoculation of breast cancer cells into the mammary fat pad, leading to development of a primary mammary tumor to the site of inoculation.
Pharmatest offers orthotopic breast cancer models with either human estrogen receptor positive MCF-7 cell line or triple negative human MDA-MB-231(SA), human BT-474 or mouse 4T1 cell lines. During the study, our experts monitor tumor growth by measuring the dimensions of the tumor at desired intervals. At the end of the experiment, we collect the primary tumor and selected organs for further analysis. In the case of the triple negative cell lines, metastasis to various distant sites, such as lungs can also be rapidly determined by ex vivo fluorescence imaging.
We can study several additional parameters, including angiogenesis, hypoxia, proliferation rate, and apoptosis, through immunohistochemistry.
Expertise in breast cancer metastasis models
Breast cancer metastasis models are suitable for testing the efficacy of drug compounds against several dissemination processes and growth in specific microenvironments. While demanding for the drug candidate, these metastasis models demonstrate excellent clinical predictivity.
Our intracardiac breast cancer bone metastasis models are clinically predictive models of cancer-induced bone disease. Radiography and dynamic fluorescence imaging are utilized to reveal the progression of the disease. Our experts analyze the data for tumor burden and, in bone metastasis studies, also for bone lesion number and area. Histological bone samples can be analyzed for tumor area, trabecular bone area and osteoclast number to reveal detailed information about the test compound’s effects on bone metastases.
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